ABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents of the rhizome of Matteuccia struthiopteris.</p><p><b>METHOD</b>The constituents were separated and purified by column chromatography with silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data.</p><p><b>RESULT</b>Six compounds were isolated and identified as demethoxymatteucinol (1), matteucinol (2), pinosylvin (3), matteuorien (4), pinosylvin 3-O-beta-D-glucopyranoside (5), matteuorienate A (6).</p><p><b>CONCLUSION</b>All Compounds were isolated from this plant for the first time.</p>
Subject(s)
Chromones , Chemistry , Dryopteridaceae , Chemistry , Flavonoids , Chemistry , Glucosides , Chemistry , Magnetic Resonance Spectroscopy , Rhizome , Chemistry , Stilbenes , ChemistryABSTRACT
To establish an HPLC-UV-ELSD method for the determination of the content of artemisinin, arteannuin B and artemisinic acid in Herba Artemisiae Annuae. The analytical column was Nucleodur RP-C18 (250 mm x 4.6 mm, 5 microm ID). The mobile phase was acetonirile-0.1% acetic acid (50: 50) and the flow rate was 1.0 mL x min(-1) with a UV detector for artemisinin, the detection wavelength at 209 nm, and the evaporative light-scattering detector (ELSD) for arteannuin B and artemisinic acid, the drift tube temperature: 50 degrees C, the nitrogen flow rate 30 psi and the gain was 50. The resolution of artemisinin, arteannuin B and artemisinic acid was good. The linear calibration curves were obtained over the range of 0.52 - 2.6 microg for artemisinin (r = 0.999 4, n = 5), 0.022 - 4.4 microg for artemisinin B (r = 0.999 9, n = 5) and 0.203 - 8.12 microg for artemisinic acid (r = 0.999 8, n = 5), separately. The mean recoveries of the three compounds were 99.45%, 102.37% and 101.10% with RSD of 2.3%, 1.7% and 0.79%, respectively. This method is simple, rapid, accurate and suitable for the determination of the content of the three compounds in the herbs.
Subject(s)
Artemisia annua , Chemistry , Artemisinins , Chromatography, High Pressure Liquid , Methods , Light , Plant Components, Aerial , Chemistry , Plants, Medicinal , Chemistry , Reproducibility of Results , Scattering, Radiation , Sensitivity and Specificity , Spectrophotometry, Ultraviolet , MethodsABSTRACT
<p><b>OBJECTIVE</b>To isolate and elucidate the constituents from stem of Chirita longgangensis var. hongyao.</p><p><b>METHOD</b>The constituents were extracted with methanol and isolated by chromatography on silica gel, Sephadex LH-20 and ODS. The structures were determined by NMR and MS spectral analysis.</p><p><b>RESULT</b>Seven compounds were identified as 2-hydroxy-7-methyl-9, 10-anthraquinone (1), 2-methyl-9, 10-anthraquinone (tectoquinone) (2), ursolic acid (3), vanillic acid (4), beta-sitosteryl-D-glucoside-6'-palmitate (5), beta-sitosterol (6), daucosterol (7), respectively.</p><p><b>CONCLUSION</b>Compounds 1, 2, 3 and 5 were isolated for the first time from the family Gesneriaceae, compounds 4 and 6 were isolated for the first time from the genus Chirita, and compound 7 was isolated for the frist time from Chirita longgangensis var.</p>
Subject(s)
Anthraquinones , Chemistry , Magnoliopsida , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , Chemistry , Triterpenes , ChemistryABSTRACT
<p><b>AIM</b>To study the chemical constituents of the rhizome of Matteuccia struthiopteris (L.) The constituents were separated and purified by column chromatography with normal Todaro.</p><p><b>METHODS</b>phase silica gel and Sephadex LH-20. Their structures were identified on the basis of physical and spectral data (MS, NMR, HMBC and HMQC).</p><p><b>RESULTS</b>Four compounds were isolated and identified as 1-O-beta-D-gl ucopyranosyl-(2S,3R,4E, 8Z) -2-N-(2'-hydroxydocosanoyl) eicosasphinga-4,8-dienine (1), 1-O-beta-D-galactosyl-(6-->1)-alpha-D-galactosyl-2,3-O-dihexadecanoyl-glycerol (2), succinic acid (3), D-mannitol (4).</p><p><b>CONCLUSION</b>Compounds 1 and 2 are new compounds. Compounds 3 and 4 were isolated from this plant for the first time.</p>
Subject(s)
Ferns , Chemistry , Galactosides , Chemistry , Glucosides , Chemistry , Glycerides , Chemistry , Mannitol , Chemistry , Molecular Conformation , Molecular Structure , Plants, Medicinal , Chemistry , Rhizome , Chemistry , Succinic Acid , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To isolate and elucidate the constituents from stem of Chirita longgangensis var. hongyao.</p><p><b>METHOD</b>The constituents were extracted with methanol and isolated by chromatography on silica gel, Sephadex LH-20 and ODS. The structures were determined by NMR and MS spectral analysis.</p><p><b>RESULT</b>Five phenylethanoid glycosides were identified as 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranoside (1), 3, 4-dihydroxyphenyl alcohol-6-O-caffeoyl--D-glucopyranoside (calceolarioside B) (2), 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranosyl-(1 --> 3)-6-O-caffeoyl-beta-D-glucopyranoside (plantainoside D) (3), 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranosyl-(1 --> 3)-4-O-caffeoyl-beta-D-glucopyranoside (plantamajoside) (4), 3, 4-dihydroxyphenyl alcohol-beta-D-glucopyranosyl-(1 --> 3)-6-O-feruloyl-beta-D-glucopyranoside (scroside E) (5), respectively.</p><p><b>CONCLUSION</b>Compounds 3 and 5 were isolated for the first time from the family Gesneriaceae, and compounds 1, 2 and 4 were isolated for the first time from the genus Chirita.</p>
Subject(s)
Catechols , Chemistry , Coumaric Acids , Chemistry , Disaccharides , Chemistry , Glucosides , Chemistry , Glycosides , Chemistry , Magnoliopsida , Chemistry , Plant Stems , Chemistry , Plants, Medicinal , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To study the chemical constituents in rhizome of Matteuccia struthiopteris.</p><p><b>METHOD</b>The compounds were isolated by normal phase silica gel chromatography. The structures were identified by physical and spectral data.</p><p><b>RESULT</b>Six compounds were isolated and identified as woodwardic acid (1), ergost-6,22-diene-3beta,5alpha,8alpha-triol (2), apigenin (3), riboflavin (4), 4-O-beta-D-glucopyranosyl-p-coumaric acid (5), 4-O-beta-D-glucopyranosylcaffeic acid (6).</p><p><b>CONCLUSION</b>All the compounds were obtained from this plant for the first time.</p>
Subject(s)
Apigenin , Chemistry , Caffeic Acids , Chemistry , Dryopteridaceae , Chemistry , Glucosides , Chemistry , Plants, Medicinal , Chemistry , Rhizome , Chemistry , Riboflavin , ChemistryABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of dihydro-qinghaosu (DQHS) on auto-antibody production, TNF alpha secretion and pathologic change of lupus nephritis in BXSB mice and the possible mechanism of DQHS in treating systemic lupus erythematosus (SLE).</p><p><b>METHODS</b>Anti ds-DNA antibody and TNF alpha in serum of the BXSB mice were detected by enzyme linked immunosorbent assay (ELISA). Renal tissue was stained by HE and Masson.</p><p><b>RESULTS</b>In the height and moderate dose DQHS groups, as compared with the model group, levels of anti-ds-DNA antibody and serum TNF alpha were significantly lower (P < 0.05); and renal pathological change was milder.</p><p><b>CONCLUSION</b>DQHS could inhibit the production of anti-ds-DNA antibody and secretion of TNF alpha and improve the pathologic lesion of lupus nephritis in BXSB mice.</p>